Biophysical evidence that connexin-36 forms functional gap junction channels between pancreatic mouse -cells
نویسندگان
چکیده
Alonso P. Moreno, Viviana M. Berthoud, Gregorio Pérez-Palacios, and E. Martha Pérez-Armendariz Krannert Institute of Cardiology, Indiana School of Medicine, Indianapolis, Indiana; Section of Hematology/Oncology, Department of Pediatrics, University of Chicago, Chicago, Illinois; and Facultad de Medicina, Departmento de Medicina Experimental, Unidad de Investigación, Enseñanza y Comunicación Ensalud Sexual y Reproductiva, Universidad Nacional Autónoma de México, Mexico City, Mexico
منابع مشابه
Biophysical evidence that connexin-36 forms functional gap junction channels between pancreatic mouse beta-cells.
Connexin-36 (Cx36) is the only gap junction protein that has been unambiguously identified in rodent pancreatic beta-cells. However, properties of gap junction channel unitary currents between beta-cells remain unrevealed. To address whether Cx36 forms functional channels in beta-cells, we characterized biophysical properties of macro- and microscopic junctional currents recorded from dual whol...
متن کاملGap junction coupling and calcium waves in the pancreatic islet.
The pancreatic islet is a highly coupled, multicellular system that exhibits complex spatiotemporal electrical activity in response to elevated glucose levels. The emergent properties of islets, which differ from those arising in isolated islet cells, are believed to arise in part by gap junctional coupling, but the mechanisms through which this coupling occurs are poorly understood. To uncover...
متن کاملConnexin diversity in the heart: insights from transgenic mouse models
Cardiac conduction is mediated by gap junction channels that are formed by connexin (Cx) protein subunits. The connexin family of proteins consists of more than 20 members varying in their biophysical properties and ability to combine with other connexins into heteromeric gap junction channels. The mammalian heart shows regional differences both in connexin expression profile and in degree of e...
متن کاملCell-free synthesis and assembly of connexins into functional gap junction membrane channels.
Several different gap junction channel subunit isotypes, known as connexins, were synthesized in a cell-free translation system supplemented with microsomal membranes to study the mechanisms involved in gap junction channel assembly. Previous results indicated that the connexins were synthesized as membrane proteins with their relevant transmembrane topology. An integrated biochemical and bioph...
متن کاملDifferent ionic selectivities for connexins 26 and 32 produce rectifying gap junction channels.
The functional diversity of gap junction intercellular channels arising from the large number of connexin isoforms is significantly increased by heterotypic interactions between members of this family. This is particularly evident in the rectifying behavior of Cx26/Cx32 heterotypic channels (. Proc. Natl. Acad. Sci. USA. 88:8410-8414). The channel properties responsible for producing the rectif...
متن کامل